RealHelix™ Superplex qPCR Kit [Probe] 의 bulk version 입니다.

대량구매를 원하시는 경우 sale@nanohelix.net 으로 문의주시기 바랍니다.

RealHelix™ Superplex qPCR Premix [Probe] 는 단일 반응으로 다양한 표적 증폭 및 검출을 수행하도록 설계되었습니다. 본 제품은 chemically modified hot-start Taq polymerase, uracil-DNA glycosylase (UDG)와 함께 buffer, Mg²⁺, dNTPs 및 안정제(stabilizers)가 혼합된 2배 농도의 premix 제품입니다. 적용된 hot-start Taq polymerase로 인하여 비특이적(non-specific) 증폭을 최소화하고 target DNA만 특이적으로 증폭할 수 있습니다. Bulk version은 premix만 제공되며, ROX reference dye는 요청 시 별도 제공됩니다.

Figure 1. Multiple amplification of five targets in a reaction by RealHelix™ Superplex qPCR Kit [Probe].

Five targets labeled with different fluorescent reporter dyes were analyzed with this Superplex qPCR Premix [Probe]. Reactions were performed using the RealHelix™ Superplex qPCR Kit [Probe] on a real-time PCR instrument, Bio-Rad CFX96.

Figure 2. Twelve-targets multiplex amplification in a reaction by RealHelix™ Superplex qPCR Kit [Probe].

Using the allele-specific FMMA probes (labeled with FAM, HEX, Texas Red, or Cy5), twelve rice SNP genotypes were identified in a single reaction. A mix of rice genomic DNAs (total 10 ng) was used for the FMMA reaction to detect all of the 12 SNPs screened for determination of rice variants. The multiplex reaction was performed with the RealHelix™ Superplex qPCR Kit [Probe] on a real-time PCR instrument, Bio-Rad CFX96.

Storage

• Store below -20 ℃
• ROX dye should be stored in a dark place.

Shelf life

• 12 months